Search results for "Citrus rootstock"

Ex vitro mycorrhization of vitro-derived plantlets of 'Carrizo' citrange [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]

2015

In this study, vitro-derived plantlets of 'Carrizo' citrange were inoculated with Glomus intraradices during the acclimatization phase, from in vitro to in vivo. Plantlets were grown in presence (+M) and in absence of inoculum (-M) and in addition, in sterile and non-sterile substrate. After three months of culture, some vegetative parameters and the level of infection, varied greatly in the different theses. In particular, the highest formation of new branched roots has been observed in inoculated plantlets cultivated in sterile substrate. Good results were observed also in non-inoculated plantlets cultivated in non-sterile substrate, in presence of endemic inoculum. The same trend was obs…

The effects of meta-Topolin and benzyladenine on in vitro organogenesis from epicotyl cuttings of Troyer citrange (Citrus sinensis[L.] Osbeck ×Poncir…

2017

Citrus tristeza virus (CTV), worldwide considered as one of the worst disease affecting Citrus plants, produced great changes in citriculture, mainly because it made mandatory the turnover of the sensitive sour orange rootstock with virus resistant ones. Among them, Troyer citrange has become one of the most employed rootstocks, due to its CTV tolerance and to the positive characteristics induced to the scion fruit production. Unfortunately, in Italy, there is a scarcity of seed mother plants. Micropropagation can be a solution for this problem. Among the in vitro techniques allowing the production of good quality true-to-type plants, the regeneration from seedling epicotyl cuttings general…

Citrus rootstock breeding: response of four allotetraploid somatic hybrids to Citrus tristeza virus induced infections

2018

Four allotetraploid somatic hybrids of citrus, with potential for rootstock improvement, have been evaluated for their response to Citrus tristeza virus (CTV) infection. CTV is the most important viral pathogen affecting citrus production worldwide. Somatic combinations of ‘Milam’ lemon (Citrus jambhiri Lush.) + Sour orange (C. aurantium L Osb.), Calamondin (C. madurensis Lour.) + ‘Keen’ sour orange (C. aurantium L.), Calamondin + ‘Femminello‘ lemon (C. limon L. Burm. F.) and Cleopatra mandarin (C. reshni Hort. ex Tan.) + ‘Femminello’ lemon, were studied. Plants were grafted with CTV-infected “Valencia” sweet orange budwood. Two different CTV strains collected in Sicily, considered as “mild…

In Vitro Plant Regeneration from Epicotyls Segments of Carrizo Citrange.

2009

STUDY ON CONVERSION OF ENCAPSULATED VITRO-DERIVED PROPAGULES OF 'C35' CITRANGE [C. SINENSIS (L.) OSB. × P. TRIFOLIATE (L.) RAF.]

2015

Fight against Citrus tristeza virus (CTV) implies the use of CTV-resistant rootstocks, such as citranges [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]. The 'C35' represents a promising tolerant to CTV genotype. The employment of innovative in vitro techniques, such as encapsulation, could open new perspectives for this genotype propagation, particularly in the nursery activity. Preliminary experiments on calcium-alginate encapsulation of microcuttings (unipolar propagules) have already been performed to evaluate the use of tissue culture for the propagation and the preservation of different citrange rootstocks: Carrizo and Troyer. In the present study, the influence of different types a…

Characterization of five sour orange clones through molecular markers and leaf essential oils analysis.

2006

Five clones of sour orange (Citrus aurantium L.) showing significant morphological differences were selected from our germplasm collection and characterized both by genetic and leaf volatiles analysis. The genetic studies were undertaken by the use of molecular markers developed by PCR-based techniques (ISSR and RAPD), while the leaf essential oil patterns were obtained by chromatographic and mass spectrometric determination. Data obtained suggest that reasonably similar information can be achieved from the two techniques, supporting each other in characterization studies.

The effects of benzyladenine and meta-Topolin on in vitro sprouting and regrowth after encapsulation of C35 citrange [Citrus sinensis (L.) Osb. × Pon…

2016

C35, a hybrid of ‘Ruby Blood’ sweet orange and trifoliate orange, is a promising Citrus rootstock, mainly due to its resistance to Citrus Tristeza Virus (CTV), one of the most serious pathology affecting citriculture. The use of resistant rootstocks, such as citranges, is an efficient tool to fight this plant disease. This implies a continuous increasing demand of resistant rootstocks, in order to carry out the turnover of sour orange, still the most used Citrus rootstock in Italy. Micropropagation, can provide innovative solutions to obtain, in a short time and in a small space, a number of plants higher than traditional propagation. Micropropagation efficiency is also affected by the cult…